Genotype-by-Environment Interaction for Quantitative Trait Loci Affecting Nitrogen Use Efficiency and Associated Traits in Potato

Abstract Deciphering the genetic basis of complex traits like nitrogen use efficiency (NUE) requires understanding genotype-by-environment (G × E) interaction and linking physiological functions agronomic to DNA markers. Multi-environment experiments were conducted in different environments representing low high levels combined with rain-fed irrigation production conditions at three locations Ethiopia: Debre-Tabor, Injibara Koga, 2013 2015. The objectives study determine G E stability genotypes for NUE potato identify markers associated NUE-related under these diverse environments. Data analyzed using GenStat, genotype plus environment (GGE) biplot model; marker-trait associations discovered R-software package GWASpoly. analysis variance that included location system had estimates (? 2 g) compared e) ge) most measured both N levels. GGE identified two mega-environments coincided systems. level Debre-Tabor Injibara, respectively, suitable discriminating cultivars being representative test evaluation mega-environment mega-environment. A total 77 marker trait traits. Multi-trait genomic regions harbored significant found on chromosomes III, V VI. effect season was greater than QTL